Resource Library

The curriculum resources listed here support the Amgen Biotech Experience. Resources are provided in partnership with Amgen, WGBH, PBS LearningMedia, Alan Marnett, and BenchFly.

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Building a Recombinant Plasmid Pasting DNA Fragments Together Building the pARA-R Plasmid Questions Glossary
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Making Sure You've Created a Recombinant Plasmid Why Do You Need To Verify LAB 4 Verification of Restriction and Ligation Using Gel Electrophoresis Questions Glossary
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Making Sure You've Got a Recombinant Plasmid Why Do You Need To Verify Verification of the Recombinant Plasmid Using Gel Electrophoresis Questions Glossary
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Getting Recombinant Plasmids Into Bacteria Transforming Bacteria with Recombinant Plasmids Transforming Bacteria with the Ligation Products Questions Glossary
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Getting Recombinant Plasmids Into Bacteria Transforming Bacteria with Recombinant Plasmids LAB 5A Transforming Bacteria with the pARA-R Plasmid Questions Glossary
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Getting Recombinant Plasmids into Bacteria Your Challenge Plasmids and Restriction Enzymes Producing Human Therapeutic Proteins in Bacteria Clone That Gene Transforming Bacteria with Recombinant Plasmids LAB 5B Transforming Bacteria with a Recombinant Plasmid (pARA-R) Questions Glossary
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Getting What We Need Producing the Protein of Interest LAB 6 Purifying the Flourescent Protein Questions Glossary
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Credits About ABE Program Introduction Program Introduction Glossary
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Khan Academy
The next steps in DNA cloning are bacterial transformation and selection. Once we have the recombinant DNA with your gene of interest, it is inserted into bacteria (transformation) and those bacteria that contain the plasmid we’re looking for are selected. We can then induce those bacteria to produce the protein we’re interested in (whether it is red fluorescent protein or a medical product such as insulin) and purify it.

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